CHST14 antibody

Catalog No. ABIN2463248

The Rabbit Polyclonal anti-CHST14 antibody has been validated for WB and ELISA. It is suitable to detect CHST14 in samples from Human, Mouse and Rat.

Quick Overview for CHST14 antibody (ABIN2463248)

Target: CHST14 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 14 (CHST14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CHST14 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHST14.

Application Details

Application Notes: CHST14 antibody can be used for detection of CHST14 by ELISA at 1:312500. CHST14 antibody can be used for detection of CHST14 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CHST14 antibody at -20 °C.

Target Details for CHST14

Target: CHST14 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 14 (CHST14))

Alternative Name: CHST14

Background: CHST14 belongs to the sulfotransferase 2 family. CHST14 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. It transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. It transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. CHST14 has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA.

Molecular Weight: 35 kDa

Gene ID: 113189

UniProt: Q8NCH0

Pathways: Glycosaminoglycan Metabolic Process